METHOD FOR PREPARING IMMOBILISED COLLAGENASE Russian patent published in 2010 - IPC C12N11/12 C12N9/48 

FIELD: medicine.

SUBSTANCE: food grade carboxymethyl cellulose is dissolved in alkalified distilled water of pH 9.5-10 and added to a filtrate of the prepared solution of collagenase recovered from crab pancreas, or of microbial collagenase recovered from Clostridium histolyticum with collagenase activity 750-1000 unit/mg and more as a powder with 1-1.5% surfactant solution added while vortexing at 3000-5000 rpm. The prepared emulsion is cooled to 5-10°C, pH of the reaction mixture is reduced to 3,0-3,5 and the mixture is kept for one day in a refrigerator. The precipitated particles are separated from the solution and an acetate buffer of pH 4-4.5 is multiply added to the stirred remained particles; then the precipitated particles containing collagenase are separated and dried up.

EFFECT: downsizing the particles of immobilised cellulase, faster enzymatic process.

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