METHOD OF LACTOFERRIN OBTAINING FROM DAIRY RAW MATERIALS Russian patent published in 2010 - IPC A23J1/20 A23J3/08 

FIELD: agriculture.

SUBSTANCE: invention refers to the dairy industry. The method of lactoferrin obtaining from dairy raw materials includes a lactoferrin chromatography from dairy raw materials on a column with a sorbent carbossimetilcellulose, balanced by 0.05 M sodium-phosphatic buffer, lactoferrin elution by 0.05 M sodium-phosphatic buffer, an ultrafiltration, salt rejection and a lactoferrin solvent lyophilising. The method differs because of the raw cow milk usage as dairy raw materials, a lactoferrin chromatography is carried out with a sorbent, by carbossimetilcellulose, balanced natrium phosphate buffer 0,05 M at pH=7.7 lactoferrin elution is carried out with natrium phosphate buffer 0.05 M at pH=7.7, in a gradient of sodium chloride concentration 0.1-1.2 M, at elution the lactoferrin solvent fractions with optical density 0.5 units and at length of a wave of 280 nanometres are gathered and then directed to the second chromatography with similar parametres, and then the allocated fractions are directed to an ultrafiltration, salt rejection and lyophilising.

EFFECT: method allows receiving native lactoferrin with cleanliness not less than 98 %.

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