FIELD: dairy industry. SUBSTANCE: milk ultrafiltrate is precipitated by saturated solution of ammonium sulfate preferably at 3-5 C, centrifuged at speed at least 12000 rpm during 0.5 to 1.5 h, and, for 10-12 h, subjected to double dialysis preferably at 7-9 C against water and sodium phosphate buffer followed by sorption of proteins and elution of protein fraction with optical density more than 0.1 at wavelength using Sephadex G-75 as sorbent. EFFECT: enhanced efficiency of isolation of anigiogenin-rich protein fraction and reduced expenses. 6 cl, 3 ex
Title | Year | Author | Number |
---|---|---|---|
METHOD OF PREPARING BIOLOGICALLY ACTIVE SUPPLEMENT "MILKANG" AND BIOLOGICALLY ACTIVE "MILKANG" PREPARED BY SAID METHOD | 2000 |
|
RU2183935C2 |
METHOD OF ANGIOGENINE ISOLATION | 1996 |
|
RU2109748C1 |
DIETARY SUPPLEMENT "MOBELIS" AND ITS PRODUCTION METHOD | 2008 |
|
RU2366294C1 |
METHOD FOR PRODUCTION OF BIOLOGICALLY ACTIVE SUPPLEMENT (BAS) FROM LAW MOLECULAR MILK CATION PROTEINS AND OBTAINED BAS | 2006 |
|
RU2318406C1 |
METHOD FOR PREPARING BIOLOGICALLY ACTIVE PROTEIN CONCENTRATE ENRICHED WITH PANCREATIC RIBONUCLEASE A, ANGIOGENIN AND LYSOZYME FROM DAIRY ULTRAFILTRATE | 2001 |
|
RU2204262C2 |
METHOD FOR PRODUCTION OF "L-PFI" DIETARY SUPPLEMENT FROM SECONDARY MILK STOCK AND "L-PFI" DIETARY SUPPLEMENT PRODUCED BY THIS METHOD | 2009 |
|
RU2400106C1 |
METHOD OF QUANTITATIVELY DETERMINING ANGIOGENIN IN BIOLOGICAL SAMPLE | 1996 |
|
RU2110066C1 |
STERILIZED ANGIOGENINE-ENRICHED MILK | 2000 |
|
RU2182791C2 |
METHOD FOR QUANTITATIVELY DETERMINING ANGIOGENINE IN MILK MATERIAL | 1998 |
|
RU2133464C1 |
METHOD OF HUMAN RECOMBINANT GAMMA-INTERFERON PREPARING | 1997 |
|
RU2132386C1 |
Authors
Dates
2001-10-27—Published
2000-06-23—Filed