METHOD FOR PREPARING THREE-DIMENSIONAL CHONDRAL GRAFT Russian patent published in 2010 - IPC A61L27/38 

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely to traumatology and orthopaedics, and can be used for correction of dystrophic and traumatic changes of cartilaginous tissue, and for early correction of growth processes in idiopathic scoliosis. The method is characterised by the fact that growth plates of newborn miniature pigs are placed in Hanks solution with cannomycin 1 g/l for 5 minutes, then growth plates are washed, crushed, transferred to test tubes with RPMI-1640 medium with 20% FBS and 1.5% collagenase of activity 240 U/ml added, and incubated; the suspension is centrifuged at 2000 rpm for 10 minutes; isolated chondroblasts are cultivated upon concentration 50-60 million; cell passage is enabled with mixed 0.25% trypsin and 0.02% EDTA; further, the cells are centrifuged at 2000 rpm for 10 minutes; then, a cell aggregate is moved to a well with nutrient medium RPMI-1640 with 10% FBS added, and cultivated for 4-6 weeks to prepare a chondral graft of 2×2×2±mm³.

EFFECT: invention prevents progressive spinal deformity at early stages, development of osteoarthrosis and osteochondrosis, reduced the postsurgical period, severely reduces number of postsurgical patient days and eliminates the staged expensive procedures on spine and joints.

1 ex, 5 dwg