FIELD: medicine.
SUBSTANCE: donor chondrocytes are produced by recovering and grinding donor cartilaginous tissue, carrying out enzymatic treatment, filtration and centrifugation of the prepared chondrocyte suspension and cultivation thereof in a nutrient medium DMEM with added 20 % FBS and perphthorane in amount 15-25 % of total amount of the nutrient medium.
EFFECT: invention allows increasing synthetic activity of chondrocytes in population in vitro, without changing substantially the other cell parameters, such as phenotype, proliferation, ability to colony-formation.
2 dwg, 1 tbl, 1 ex
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