FIELD: chemistry; biochemistry.
SUBSTANCE: invention relates to biotechnology and is a method of detecting living cells of a microorganism through differentiation of living cells from dead cells or damaged cells in a test specimen. The method involves a step for treating the test specimen with topoisomerase poison or DNA-gyrase poison, a step for extracting DNA from the test specimen and amplification of the target section of the extracted DNA through a polymerase chain reaction (PCR), where the length of the target section is between 900 and 3000 nucleotides, and a step for analysing the amplification product.
EFFECT: invention enables high-accuracy determination of presence of living organisms.
52 cl, 18 dwg, 2 tbl, 4 ex
