FIELD: medicine.
SUBSTANCE: according to the following stages, flow cytometry is used to detect living cells, damaged cells, VNC cells and dead cells of a microorganism in a tested sample: a) the stage of processing the tested sample with enzyme chosen from lipolytic enzymes and protease with activity to destruct the cells differing from those of the microorganism, colloid protein particles or lipids found in the analysed sample; b) the stage of processing the tested sample with topoisomerase inhibitor and/or DNA-gyrase inhibitor; e) the stage of processing the tested sample been processed at the stages a) and b) with a kernel-dyeing agent, and d) the stage of detecting microorganisms in the tested sample processed by the kernel-dyeing agent with using flow cytometry.
EFFECT: convenient and fast detecting of live microorganisms and identification of the damaged and dead cells in foodstuff and clinical samples.
19 dwg, 8 tbl, 8 ex
| Title | Year | Author | Number |
|---|---|---|---|
| MICROORGANISM DETECTION METHOD AND MICROORGANISM DETECTION SET | 2006 |
|
RU2395583C2 |
| METHOD AND KIT FOR DETECTION OF MICROORGANISMS | 2010 |
|
RU2527897C2 |
| METHOD AND SET FOR MICROORGANISM DETECTION | 2008 |
|
RU2410440C1 |
| RECOMBINANT PLASMID DNA pQE-70_LysAP46 PROVIDING SYNTHESIS OF RECOMBINANT PROTEIN LysAP46, STRAIN OF ESCHERICHIA COLI BACTERIA - PRODUCER OF RECOMBINANT PROTEIN LysAP46 AND RECOMBINANT PROTEIN LysAP46, WHICH HAS ANTIBACTERIAL EFFECT | 2022 |
|
RU2809842C1 |
| NON-REPLICATING PROBIOTIC MICROORGANISMS PROTECTING CHILDREN FROM GASTROINTESTINAL INFECTIONS | 2011 |
|
RU2568830C2 |
| FROZEN CONFECTIONS CONTAINING PROBIOTIC MICRO-ORGANISMS | 2011 |
|
RU2593716C2 |
| NON-REPLICATING PROBIOTIC MICROORGANISMS PROTECTING AGAINST INFECTIONS OF UPPER AIRWAY | 2011 |
|
RU2589706C2 |
| DOMESTIC ANIMAL FODDERS CONTAINING PROBIOTIC MICROORGANISMS | 2011 |
|
RU2580881C2 |
| METHOD FOR DETECTING CONTENT OF LIVE MICROORGANISMS | 0 |
|
SU1018970A1 |
| METHOD FOR DETECTING DAMAGES TO MICROORGANISMS | 0 |
|
SU1096280A1 |
