METHOD AND SET FOR MICROORGANISM DETECTION Russian patent published in 2011 - IPC C12Q1/68 

FIELD: chemistry; biochemistry.

SUBSTANCE: invention relates to microbiology. Described is a method for detecting living cells of microorganisms in a test sample, involving adding a cross-linking agent to the test sample, capable of cross-linking DNA when exposed to light having wavelength 350-700 nm; exposure to light having wavelength 350-700 nm; removing the cross-linking agent; adding medium and further incubation; adding cross-linking agent capable of cross-linking DNA when exposed to light having wavelength 350-700 nm to the incubated test sample once more; repeated exposure to light having wavelength 350-700 nm; extraction of DNA from this test sample and amplification of the target region of the extracted DNA and analysis of the amplified product. Disclosed is a method of detecting living cells of microorganisms in a test sample, which involves using a set containing the following components: cross-linking agent capable of cross-linking DNA when exposed to light having wavelength 350-700 nm; medium and primer(s) for amplifying the target region of the DNA of the microorganism which is the detection target using a nucleic acid amplification method.

EFFECT: invention enables detection of living cells of microorganisms in a test sample.

26 cl, 5 dwg, 17 tbl, 5 ex