FIELD: medicine.
SUBSTANCE: offered invention can be used for the quantitative determination of alive bacteria. The offered method involves PCR-based product amplification with using the primers able to specific hybridisation with rRNA of a bacterium of interest. It is followed with the analysis of cell number of the alive bacterium of interest taking into account PCR cycle number.
EFFECT: invention allows precise determination of cell number of the alive bacterium of interest in a test sample.
8 cl, 7 dwg, 4 tbl, 10 ex
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