METHOD OF PREPARING SOLID CULTURE MEDIUM Russian patent published in 2010 - IPC C12N1/00 

Abstract RU 2396335 C1

FIELD: chemistry; biochemistry.

SUBSTANCE: invention relates to biotechnology and can be used in practice in microbiological laboratories. The method involves adding a thickener to liquid culture substrate, adding ingredients in accordance with the standard formula of the medium and sterilisation in an autoclave. Molten medium cooled to 65°C is mixed with 0.2-0.3 wt % prepared emulsion of a mixture of perfluoro-organic compounds in ratio of 3:1, perfluoro-1,3-dimethylcyclohexane and perfluorodecalin with average size of emulsified particles equal to 90-270 nm.

EFFECT: invention leads to increase in germination of the inoculated microorganisms, increase in growth intensity and yield of the bacterial culture.

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RU 2 396 335 C1

Authors

Bakulin Mikhail Konstantinovich

Borisevich Igor' Vladimirovich

Kovtun Anatolij Leonidovich

Alekseev Sergej Mikhajlovich

Kuznetsov Sergej Leonidovich

Grudtsyna Anna Stanislavovna

Darmova Svetlana Vladimirovna

Bakulin Vladimir Mikhajlovich

Polishchuk Vitalij Ivanovich

Dates

2010-08-10Published

2009-03-18Filed