METHOD OF DETECTING PATHOGENIC MICROORGANISMS Russian patent published in 2010 - IPC C12N1/02 B82B1/00 

FIELD: chemistry; biochemistry.

SUBSTANCE: microorganism is conjugated with nanoparticles of a magnetic substance in the analysed medium with subsequent concentration of the conjugates and determination of presence and concentration of microorganisms using a diagnostic mark. Nanoparticles of transition metals or their compounds simultaneously serve as the magnetic substance and the diagnostic mark. Before concentration of labelled conjugates from the analysed medium, nanoparticles which are not bonded to microorganisms are extracted. The labelled conjugates are concentrated by forming an immunocomplex on a solid chemically inert carrier: antibody microorganism labelled with a magnetic mark, with subsequent extraction of the immunocomplex from the medium on the carrier. Presence and concentration of microorganisms is determined from a signal generated by transition metal ions obtained through chemical decomposition of the immonocomplex.

EFFECT: method is aimed at increasing sensitivity and reliability of analysis, and widening the range of determined.

6 cl, 9 dwg, 8 ex