ELECTROCHEMICAL IMMUNOASSAY METHOD FOR MICROORGANISM TEST Russian patent published in 2015 - IPC C12N1/02 C12Q1/04 G01N33/53 B82B1/00 

FIELD: medicine.

SUBSTANCE: invention refers to biotechnology, particularly to a microorganism test in various objects and media. The method provides conjugating electrically Fe⁰, MgFe₂O₄ or Fe₃O₄ labelled bacteria in an aqueous medium at specified parameters. Unbounded nanoparticles are separated with using a magnetic field, and a working electrode made of gold, platinum or graphite-bearing materials and having a surface pre-modified by antibodies specific to a bacterial strain to be tested is immersed into an analysed solution. The electrode is kept at the specified parameters to form an immune complex on its surface and washed in a buffer solution containing normal horse serum and Tween-20. The electrode is removed from the solution and placed into a cell containing LiClO₄, dissolved in acetonitrile, dimethyl formamide or dimethyl sulphoxide; the bacterial count is determined by a value of analytical oxidation of the nanoparticles localised in the immune complex on the surface of the working electrode.

EFFECT: invention enables providing higher analysis sensitivity, better output and simplified analysis procedure.

7 dwg, 6 ex