METHOD OF DETERMINING THE CONTENT OF BACTERIA IN THE ANALYZED MEDIUM USING MAGNETITE NANOPARTICLES Russian patent published in 2023 - IPC C12Q1/06 C12N1/02 B82Y5/00 

FIELD: biotechnology.

SUBSTANCE: method of determining the content of bacteria in the analyzed medium using magnetite nanoparticles is proposed, the method includes mixing of a sample of the analyzed medium with a pre-synthesized Fe₃O₄-antibody immunoconjugate, forming an immunocomplex in an aqueous medium at the temperature of 37°C, incubation for 5 min followed by exposure to a permanent magnet with an area of 1 cm² with a magnetic field strength of 31.83⋅10³ A/m for 1 minute and adding excess mineral acid to reach pH≤2. The content of bacteria is determined by the magnitude of the analytical signal, which is the electrochemical response of the reduction of Fe₃O₄ nanoparticles in the potential range from +0.5 V to -0.1 V.

EFFECT: invention provides an extension of the arsenal of methods of determining the content of bacteria in samples of various compositions.

1 cl, 1 dwg, 3 ex