FIELD: microbiology, biotechnology, medicine, in particular production of living vaccines against viral infections.
SUBSTANCE: invention relates to living vaccines for rectal administration against viral infections based on attenuated recombinant Salmonella strains bearing protective viral antigens. Suppository for immunoprophylaxis of viral infections contains (per one 2 g suppository, mass %): cell slurry of attenuated recombinant Salmonella strains transformed with pGEX-2T-TBI, pcDNA-TCI, or pKHBc bearing gene of protective viral antigens, mixed with suppository base in amount of 106-109 living cells 1 %; fatty base 94 %; and emulsifier T2 5 %. As fatty solid cooking oil (94 %); or hydrolyzed cotton oil (94 %); or mixture of cooking oil (60 %), Cocoa fat (24 %), and solid wax (10 %).
EFFECT: agent for inducing of humoral and cell immune response to respective viral antigen.
8 tbl, 11 ex, 3 dwg
| Title | Year | Author | Number |
|---|---|---|---|
| RECOMBINANT ATTENUATED STRAIN OF BACTERIUM SALMONELLA ENTERIDITIS E-23/PGEX-2T-TBI AS CANDIDATE FOR CONSTRUCTION OF LIVE VACCINE AGAINST HUMAN IMMUNODEFICIENCY VIRUS | 2002 |
|
RU2237089C2 |
| RECOMBINANT PLASMID DNA PCDNA-TCI PROVIDING EXPRESSION OF ARTIFICIAL GENE TCI IN EUCARYOTIC CELLS AND RECOMBINANT ATTENUATED STRAIN OF BACTERIUM SALMONELLA ENTERITIDIS E-23 PC-DNA-TCI AS CANDIDATE FOR CONSTRUCTION OF LIVE DNA-VACCINE AGAINST HUMAN IMMUNODEFICIENCY VIRUS | 2003 |
|
RU2248396C2 |
| RECOMBINANT ATTENUATED STRAIN OF BACTERIUM SALMONELLA TYPHIMURIUM T10/PKHBC AS PRODUCER OF HEPATITIS B VIRUS CORE ANTIGEN | 2002 |
|
RU2216590C1 |
| RECOMBINANT VACCINE AGAINST HUMAN IMMUNODEFICIENCY VIRUS TYPE 1 | 2006 |
|
RU2317107C2 |
| STRAIN OF BACTERIUM SALMONELLA ENTERIDITIS E-23 BMC 120 FOR IMMUNIZATION AGAINST HUMAN IMMUNODEFICIENCY VIRUS | 2001 |
|
RU2192277C1 |
| RECOMBINANT PLASMIDE DNA pU8HBc-preS1, ENCODING PROTEIN GENE OF HEPATITIS B VIRUS CORE ANTIGEN INCLUDING pre-S1 REGION EPITOPE OF HEPATITIS B VIRUS SURFACE PROTEIN AND BACTERIUM STRAIN Escherichia coli AR PRODUCER OF HYBRID HBc-preS1 PROTEIN | 2005 |
|
RU2295570C2 |
| VACCINE AGAINST VIRAL INFECTIONS | 2000 |
|
RU2217162C2 |
| ASSOCIATED VACCINE FOR PREVENTING DISEASES IN FURRED ANIMALS | 2001 |
|
RU2194530C1 |
| ARTIFICIAL PROTEIN-IMMUNOGEN TCI COMPRISING MULTIPLE CTL-EPITOPES OF HIV-1 BASIC ANTIGENS, ARTIFICIAL GENE TCI ENCODING POLYEPITOPE PROTEIN-IMMUNOGEN TCI | 2002 |
|
RU2238946C2 |
| COMPOSITION OF VACCINES WITH DOUBLE ADJUVANT, PREPARATION AND USE | 2015 |
|
RU2729646C2 |
