FIELD: medicine.
SUBSTANCE: level of LP(a) is determined by additional processing of 0.3 ml of blood serum with 0.1% solution of Triton X-100 with 15 min incubation at 20°C. After that blood serum is incubated with solution of Sudan B for 1 hour in dark thermostat at 40°C and sample is introduced into hole in agarose gel with area 4×20 mm for LP electrophoresis with further fixation of electroforegrams, their drying, densitometry. In case of 40% or higher reduction of LP(a) level in comparison with initial level treatment of coronary heart disease is estimated as efficient.
EFFECT: increased accuracy of predicting coronary heart disease.
2 ex, 6 dwg
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