DIFFERENTIAL DIAGNOSTIC TECHNIQUE FOR HERPETIC INFECTIONS OF GASTRIC MUCOSA OR DENTAL PULP Russian patent published in 2010 - IPC A61B10/02 G01N1/28 

FIELD: medicine.

SUBSTANCE: differential diagnostics of herpetic infections of gastric mucosa us membranes of a stomach or dental pulp is ensured by coordination marking of a biopsy material. Histologic sections of thickness no more than 3 mcm are prepared. The prepared sections are applied on slides as follows: the first section is arranged on the first slide, the second section is applied on the second, the third slide is used for the third section, the fourth section is placed on the first slide and further the same sequence, the number of slides is determined by the identified virus count. Each preparation is analysed for specific viruses of the herpes groups by immunohistochemistry with using specific monoclonal antibodies. The results of each reaction are recorded by the presence of specifically stained antigen granules in a histologic sample, and the images of the results of each reaction are saved in the electronic form. The formed images of the following sections are aligned by the coordination mark. If observing the specifically stained antigen granules of various localisation, herpetic mixed infection of gastric mucosa and dental pulp is diagnosed. When observing the specifically stained antigen granules of the same localisation herpetic monoinfection with immune cross-reaction is diagnosed.

EFFECT: technique presents higher accuracy of diagnosing herpetic infections of gastric mucosa or dental pulp ensured by differential diagnostics between mixed infection and monoinfection with immune cross-reaction.

3 dwg, 3 ex