ARTIFICIAL GENE, CODING CIMERIC PROTEIN OF HUMAN ANGIOGENIN, CHIMERIC PLASMID pJZZ-A, ENSURING EXPRESSION OF GENE OF CHIMERIC PROTEIN OF HUMAN ANGIOGENIN IN Escherichia coli AND STRAIN OF Escherichia coli BL21(DE3)/pJZZ-A-PRODUCENT OF RECOMBINANT CHIMERIC PROTEIN OF HUMAN ANGIOGENIN Russian patent published in 2014 - IPC C07K14/515 C07K19/00 

Abstract RU 2512527 C2

FIELD: chemistry.

SUBSTANCE: invention relates to biotechnology, in particular to genetic engineering Claimed is artificial gene, which codes chimeric protein of human angiogenin Recombinant plasmid pJZZ-A, representing vector pJexpress414 and containing said gene, embedded on sites NdeI/XhoI, is described Also claimed is recombinant strain of E coli BL21(DE3)/pJZZS-A.

EFFECT: invention makes it possible to increase efficiency of hybrid gene expression and increase production of recombinant chimeric protein of human angiogenin

3 cl, 3 dwg, 1 tbl

Similar patents RU2512527C2

Title Year Author Number
RECOMBINANT PLASMID FOR EXPRESSION IN PICHIA PASTORIS YEAST GENE OF CHIMERIC PROTEIN OF HUMAN ANGIOGENIN AND STRAIN OF PICHIA PASTORIS YEAST - PRODUCER OF RECOMBINANT CHIMERIC PROTEIN OF HUMAN ANGIOGENIN 2017
  • Purtov Aleksej Anatolevich
  • Mamaev Aleksej Lvovich
RU2658758C1
CHIMERA PLASMID PZZSA FOR EXPRESSION OF CHIMERA PROTEIN ANGIOGEN AND STRAIN OF ESCHERICHIA COLI BL21(DE3) PZZSA AS PRODUCER OF RECOMBINANT CHIMERA HUMAN PROTEIN ANGIOGEN 2002
  • Mertvetsov N.P.
  • Ramazanov Ju.A.
  • Majstrenko V.F.
RU2221043C2
RECOMBINANT PLASMID DNA ENCODING HYBRID POLYPEPTIDES WITH PROPERTIES OF RED FLUORESCENT PROTEIN mCherry TO PRODUCE HYBRID FLUORESCENT PROTEINS IN Escherichia coli 2013
  • Petrovskaja Lada Evgen'Evna
  • Shingarova Ljudmila Nikolaevna
  • Gapizov Sultan Shakhbanovich
  • Krjukova Elena Aleksandrovna
  • Boldyreva Elena Filippovna
  • Jakimov Sergej Aleksandrovich
  • Dolgikh Dmitrij Aleksandrovich
  • Kirpichnikov Mikhail Petrovich
RU2527171C1
RECOMBINANT PLASMID DNA PES1-6 ENCODING POLYPEPTIDE SOMATOTROPIN AND STRAIN ESCHERICHIA COLI BL21(DE3)/PES1-6 AS PRODUCER OF RECOMBINANT SOMATOTROPIN 2002
  • Gabibov A.G.
  • Ponomarenko N.A.
  • Vorob'Ev I.I.
  • Demin A.V.
  • Mart'Janov V.A.
  • Shuster A.M.
  • Bairamashvili D.I.
  • Miroshnikov A.I.
RU2233879C1
RECOMBINANT PLASMID DNA PES3-7 ENCODING PEPTIDE WITH SEQUENCE OF HUMAN GRANULOCYTE COLONY-STIMULATING FACTOR (G-CSF) AND STRAIN ESCHERICHIA COLI BL21(DE3)/PES3-7 AS PRODUCER OF HUMAN RECOMBINANT G-CSF 2003
  • Gabibov A.G.
  • Ponomarenko N.A.
  • Vorob'Ev I.I.
  • Demin A.V.
  • Mart'Janov V.A.
  • Shuster A.M.
  • Bairamashvili D.I.
  • Miroshnikov A.I.
RU2260049C2
RECOMBINANT PLASMID DNA pET22b(+)/SLURP-1 CODING SLURP-1 PROTEIN, AND STRAIN OF BACTERIA Escherichia coli BL21(DE3)/pET22b(+)/SLURP-L-PRODUCENT OF HUMAN SLURP-1 PROTEIN 2010
  • Shulepko Mikhail Anatol'Evich
  • Ljukmanova Ekaterina Nazymovna
  • Lobas Anna Aleksandrovna
  • Dolgikh Dmitrij Aleksandrovich
  • Kirpichnikov Mikhail Petrovich
RU2453602C2
RECOMBINANT PLASMID DNA pES6-1 ENCODING HUMAN POLYPEPTIDE INTERFERON BETA-1b AND STRAIN Escherichia coli BDEES6 AS PRODUCER OF HUMAN RECOMBINANT INTERFERON BETA-1b 2004
  • Bairamashvili D.I.
  • Vorob'Ev I.I.
  • Gabibov A.G.
  • Demin A.V.
  • Miroshnikov A.I.
  • Mart'Janov V.A.
  • Ponomarenko N.A.
  • Shuster A.M.
RU2261913C1
RECOMBINANT PLASMID DNA PF265 CODING HYBRID POLYPEPTIDE CONTAINING HUMAN PROINSULIN, AND BACTERIAL STRAIN ESCHERICHIA COLI - PRODUCER OF HYBRID POLYPEPTIDE CONTAINING HUMAN PROINSULIN 2019
  • Latypov Vitaliy Felixovich
  • Kornakov Igor Aleksandrovich
  • Robustova Svetlana Eduardovna
  • Khomutova Oksana Sergeevna
  • Rodionov Petr Petrovich
RU2728611C1
RECOMBINANT PLASMID DNA PES4-4 ENCODING HUMAN POLYPEPTIDE INTERFERON ALPHA-2B AND STRAIN ESCHERICHIA COLI BREES4 AS PRODUCER OF RECOMBINANT HUMAN INTERFERON ALPHA-2B 2003
  • Bairamashvili D.I.
  • Vorob'Ev I.I.
  • Gabibov A.G.
  • Demin A.V.
  • Miroshnikov A.I.
  • Mart'Janov V.A.
  • Ponomarenko N.A.
  • Shuster A.M.
RU2258081C1
RECOMBINANT PLASMID DNA pET22b(+)/slurp-2 CODING SLURP-2 PROTEIN, AND STRAIN OF BACTERIA Escherichia coli BL21(DE3) pET22b(+)/slurp-2 PRODUCER OF HUMAN SLURP-2 PROTEIN 2015
  • Ljukmanova Ekaterina Nazymovna
  • Shulepko Mikhail Anatolevich
  • Shenkarev Zakhar Olegovich
  • Dolgikh Dmitrij Aleksandrovich
  • Kirpichnikov Mikhail Petrovich
RU2583307C2

RU 2 512 527 C2

Authors

Ramazanov Jurij Akhmetovich

Beklemishev Anatolij Borisovich

Dates

2014-04-10Published

2012-07-10Filed