FIELD: medicine.
SUBSTANCE: method of producing 1,3-propanediol with application of raw glycerol, by-product of biodiesel fuel production, includes inoculation of strain of 1,3-propanediol-producing bacteria, into medium of inoculation culture. Inoculation culture is added into enzymatic medium, which contains raw glycerol, and periodic fermentation in anaerobic and aerobic conditions is carried out at temperature from 30°C to 37°C. Raw glycerol, or mixture of raw glycerol and glucose is additionally supplied into enzymatic medium, on condition that, concentration of glycerol in enzymatic medium is supported in the range from 10 to 40 g/l or ratio of concentration of glycerol to glucose in mixture constitutes 8~10:1. 1,3-propanediol is isolated and purified. 1,3-propanediol mole output constitutes 0.45-0.6.
EFFECT: method allows to increase efficiency of using by-product of biodiesel fuel production.
10 cl, 1 dwg, 1 tbl, 4 ex
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Authors
Dates
2010-11-20—Published
2006-06-01—Filed