FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology and constitutes a method for producing 1,3-propanediol by fermentation of a recombinant microorganism, capable of overexpressing the acetyl-CoA carboxylase genes accBC and accDA; the malonyl-CoA synthetase gene mcr; the 3-hydroxypropionyl-CoA synthetase gene pcs, the 3-hydroxypropionyl-CoA reductase gene pduP, and the 1,3-propanediol oxidoreductase gene yqhD. The recombinant microorganism is subjected to fermentation in a fermentable culture in a flask or in a fermenter using glucose as a raw material to obtain 1,3-propanediol. The recombinant microorganism can utilise glucose, sucrose, molasses, xylose, etc. as raw materials in the process of fermentation without adding high-priced vitamin B12.
EFFECT: invention provides a possibility to produce 1,3-propanediol with a high degree of efficiency.
10 cl, 1 dwg, 4 ex
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