METHOD OF ASSESSMENT OF LOCAL INFLAMMATION ACTIVITY WITH UNDERLYING UTERINE CERVICAL DISEASES Russian patent published in 2010 - IPC G01N33/48 

FIELD: medicine.

SUBSTANCE: assessment of local inflammation activity with underlying uterine cervical diseases involves the collection of biomaterial samples to be analysed by delivering a native material sampling agent to the external orifice of uterus to a nidus surface directly without introducing a sampling agent inside the cervical canal. The collected material is fully immersed in a test tube filled with 1.0 ml of 0.155 M saline solution and agitated in a vortex centrifuge for 10 minutes. The prepared materials are investigated by enzyme-linked immunosorbent assay (ELISA) and analysed for cytokine interleukin-1β concentration by spectrophotometric method. The derived value within 70 pg/ml to 150 pg/ml enables to observe the presence of a chronic inflammatory process, while the value exceeding 150 pg/ml shows the presence of an active inflammatory process and expressing immune response.

EFFECT: method allows eliminating injuries of the mucosa and improving the quality of the investigated native material without complicating the investigation process.

3 cl, 3 ex