METHOD FOR PRODUCING SERUM FOR TREATMENT AND PREVENTION OF VIRAL INTESTINAL AND RESPIRATORY DISEASE IN CALVES Russian patent published in 2010 - IPC A61K39/12 A61P31/12 

FIELD: medicine, veterinary science.

SUBSTANCE: invention refers to veterinary virology. The method consists in the fact that antigens are produced with the use of bovine rednose virus of strain MVA (1)80 No.15 of infective activity 7.0-8.0 lg TCC₅₀/cm³, bovine parainfluenza-3 virus of strain MVA (1)77 No.14 of infective activity 6.0-7.0 lg TCC₅₀/cm³, bovine diarrhoeia - mucous disease virus of strain VK1-V1 genotype 1 of infective activity 4.5-5.5 lg TCC₅₀/cm³, bovine rotavirus of strain RP-82 of infective activity 5.5-6.5 lg TCC₅₀/cm³, bovine coronavirus of strain KP-83 of infective activity 5.0-6.5 lg TCD₅₀/cm³. The viruses are purified and concentrated by adding barium sulphate in the final concentration 5-10%. A sedimented sorbent is separated by centrifugation. The virus is eluted from the sorbent by adding 0.2-0.25 M of sodium citrate. The prepared antigens are mixed in the volume ratios 1:(0.8-1.2):(0.8-1.2):(0.8-1.2):(0.8-1.2)):(0.8-1.2). Formalin in the final concentration 2.5-3.0% is added to bovine adenovirus of strain B-10 serotype 1 of infective activity 5.0-6.5 lg TCC₅₀/cm³ and incubated for 24-30 hours at 36.5-37.5°C. Then aluminium hydrate is added to produce a bovine adenovirus strain V-10 serotype 1 antigen. Then, the producers are triply hyperimmunized separately with the mixed antigens and bovine adenovirus strain V-10 serotype 1 antigen in two points. On the 7-10^th post-hyperimmunisation day, the producers' blood serum is analysed for antibody titres. The producers' blood is drawn in certain antibody titres. Then, the producers' serums prepared with the mixed virus antigens are combined with the serum prepared for the bovine adenovirus strain V-10 serotype 1 antigen at 1:(0.8-1.2) respectively. The end product is preserved by adding merthiolate in the final concentration 0.01-0.02 %.

EFFECT: method allows effective prevention of calves' viral intestinal and respiratory infections, reduced treatment length in the infected calves with their survival rate increased by 25-30 %.

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