METHOD OF DIFFERENTIAL DIAGNOSTICS OF BULLOUS DERMATOSES Russian patent published in 2011 - IPC A61B10/00 G01N1/30 

FIELD: medicine.

SUBSTANCE: invention relates to medicine, namely to dermatovenerology. In order to carry out differential diagnostics of bullous dermatoses level of blister formation in skin is determined. Erosion on skin is processed with acridine orange in dilution 1:5000 on physiological solution and examined by means of frontal lens of contact 60 fold magnification objective. If blister bottom is represented by cells of granular epidermis layer, the cells being large, horizontally oriented with nuclei of various size, configuration and light intensity with cytoplasm in granular layer cells having yellowish glow and nuclei with signs of lysis, disintegration of epidermis cells is detected, in blister cavity there are acantolytic cells with large nuclei and glowing red cytoplasm, seborrheal pemphigus is diagnosed. If bottom of erosion is represented by cells of basal layer, and in cells there are large nuclei with intensive green glow, acantolytic cells have a large nucleus and intensively glowing red cytoplasm and when lens of objective touches the erosion bottom disintegration of epidermis cells is detected and its strata with acantolytic cells are freely floating in tissue fluid, pemphigus vulgaris is diagnosed. If destruction of basal membrane is observed, blister bottom is represented by papillary layer of derma, cell infiltrate, where non-fluorescent dark papillary is detected capillaries are contouring, Duhring's dermatitis herpetiformis is diagnosed.

EFFECT: method ensures high accuracy of diagnostics at early stages of bullous dermatites during short period of time.

7 dwg, 3 ex