FIELD: medicine. SUBSTANCE: method involves preparing 5 mcm thick slices of unfixed tissue in cryostat at -20 C, treating with 40-60% water solution of ethanol, washing in physiological salt solution of 7.0-7.4 pH during 110 min to remove proteins not bound to the tissue, treating the slices with luminescent serum anti-immunoglobulin G during 30 min, washing during 10 min and putting into neutral glycerol. Cryostatic media of patient organs are investigated to detect presence of immunoglobulin G in intercellular substance of multilayer flat epithelium and making pemphigus diagnosis. EFFECT: enhanced reliability of diagnosis. 1 tbl
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