FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology and molecular genetics. Disclosed is genetic construct for heterologous expression of a thiazole-oxazole modified peptides of clebsazolin in cells of E. coli bacteria on the basis of two inducible expression vector plasmids - pBAD His/B and pET28a. Invention also relates topreparation agent thiazole-oxazole modified peptides of cleftsazolin by cultivating producer strain bacteria E. coli, obtained by transformation of bacterial cells by said genetic construct, followed by isolation of peptide from culture medium.
EFFECT: invention enables to obtain thiazole-oxazole modified peptide of klebsazolitsin possessing antibacterial action due to inhibition of early stage of protein synthesis.
3 cl, 14 dwg
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