FIELD: chemistry.
SUBSTANCE: during immunoenzymometric analysis, a solid-phase antigen is immobilised on the surface of a solid phase. Further, a sample and a preparation which contains specific antigens with which free mycotoxin and the solid phase compete to bind are added. An anti-specific immunoenzymometric conjugate is then added and finally results are evaluated using a substrate-indicator mixture. The solid-phase antigen used is a conjugate of T-2 mycotoxin with an antigen of fraction 1 of a pestilential microbe. Rabbit hyperimmune serum is used at the competitive binding step.
EFFECT: invention increases sensitivity and specificity during detection of T-2 mycotoxin in liquid samples.
2 ex
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Authors
Dates
2011-02-20—Published
2009-09-07—Filed