METHOD FOR IMMUNOENZYMOMETRIC DETECTION OF T-2 MYCOTOXIN IN LIQUID SAMPLES Russian patent published in 2011 - IPC G01N33/53 C12Q1/28 

Abstract RU 2412443 C1

FIELD: chemistry.

SUBSTANCE: during immunoenzymometric analysis, a solid-phase antigen is immobilised on the surface of a solid phase. Further, a sample and a preparation which contains specific antigens with which free mycotoxin and the solid phase compete to bind are added. An anti-specific immunoenzymometric conjugate is then added and finally results are evaluated using a substrate-indicator mixture. The solid-phase antigen used is a conjugate of T-2 mycotoxin with an antigen of fraction 1 of a pestilential microbe. Rabbit hyperimmune serum is used at the competitive binding step.

EFFECT: invention increases sensitivity and specificity during detection of T-2 mycotoxin in liquid samples.

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RU 2 412 443 C1

Authors

Dubrovin Mikhail Jur'Evich

Kytmanov Aleksej Aleksandrovich

Bakulina Ljudmila Vladimirovna

Vorob'Ev Konstantin Anatol'Evich

Darmov Il'Ja Vladimirovich

Dates

2011-02-20Published

2009-09-07Filed