FIELD: medicine.
SUBSTANCE: invention represents recombinant thermostable DNA-ligase Acidilobus sp 1904, demonstrating ligase activity in presence of magnesium ions, in the range of pH values from 6.8 to 7.4, sodium chloride concentrations from 0 to 100 mM. DNA-ligase consists of 607 amino acids and has weigh 68 kDa, determined by method of electrophoresis in polyacryl gel and has amino acid sequence SEQ ID N0:2. Invention also relates to DNA, coding DNA-ligase Acidilobus sp 1904, and method of obtaining said ligase.
EFFECT: invention makes it possible to extend assortment of DNA-ligases.
3 cl, 4 dwg, 6 ex
| Title | Year | Author | Number |
|---|---|---|---|
| THERMOSTABLE DNA-LIGASE OF THERMOCOCCUS ARCHAEA, METHOD FOR PRODUCING THEREOF AND DNA NUCLEOTIDE SEQUENCE CODING SUCH DNA-LIGASE | 2009 |
|
RU2405823C2 |
| USE OF RECOMBINANT BETA-GALACTOSIDASE AsBgl 1390 FROM ARCHAEA SACCHAROVORANS AS BETA-GLUCOSIDASE, BETA-XYLOSIDASE AND BETA-MANNOSIDASE | 2012 |
|
RU2523581C2 |
| STRAIN OF Esherichia coli EX pQE30 BACTERIUM, PRODUCER OF ENDOXYLANASE OF Geobacillus stearothermophillus 22 BACTERIUM | 2013 |
|
RU2542486C1 |
| RECOMBINANT PLASMID pESAT6-CFP10-DBD, RECOMBINANT STRAIN Escherichia coli M15 [pREP4, pESAT6-CFP10-DBD], METHOD FOR PREPARING, IMMOBILISING, CONCENTRATING AND PURIFYING RECOMBINANT PROTEIN ESAT6-CFP10-DBD ON DEXTRAN, RECOMBINANT PROTEIN ESAT6-CFP10-DBD AND IMMUNOGENIC COMPOSITION CONTAINING PROTEIN ESAT6-CFP10-DBD | 2013 |
|
RU2539026C1 |
| METHOD FOR PREDICTING TUBERCULOUS INFECTION, RECOMBINANT PLASMID DNA CODING THE SYNTHESIS OF CFP10-ESAT6 HYBRID PROTEIN OUT OF MYCOBACTERIUM TUBERCULOSIS, THE METHOD OF ITS OBTAINING, CFP10-ESAT6 HYBRID PROTEIN AND ITS APPLICATION | 2003 |
|
RU2277540C2 |
| RECOMBINANT PLASMID DNA FOR DETECTION OF AGENTS DAMAGING GENETIC APPARATUS OF CELL (VARIANTS) | 2005 |
|
RU2311459C2 |
| RECOMBINANT PLASMID DNA pQE30/derf2L CODING PROTEIN der f 2L OF MITE Dermatophagoides farinae, AND BACTERIAL STRAIN Escherichia coli M15/pQE30/derf2L - PRODUCER OF SUCH PROTEIN | 2012 |
|
RU2522817C9 |
| PEPTIDE PURINOCEPTOR MODULATOR | 2010 |
|
RU2422459C1 |
| RECOMBINANT CHIMERIC REVERSE TRANSCRIPTASE EXHIBITING INCREASED PROCESSIVITY AND RESISTANCE TO AMPLIFICATION INHIBITORS, AND METHOD FOR PRODUCTION THEREOF | 2020 |
|
RU2762291C1 |
| RECOMBINANT PUSB2-AMQ PLASMID SYNTHESISING PROTEIN OF BACILLUS AMYLOLIQUEFACIENS ALPHA-AMYLASE AND BACILLUS SUBTILIS/PUSB2-AMQ STRAIN - PRODUCER OF PROTEIN OF BACILLUS AMYLOLIQUEFACIENS ALPHA-AMYLASE | 2020 |
|
RU2747627C1 |
