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2 405 823

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IPC

C12N15/00(2006-01-01)

(21) (22)

Application

2009102855/10, 2009-01-29

(24)

Start date

2009-01-29

(22)

Actual filing date

2009-01-29

(45)

Published

2010-12-10

(72)

Inventor

Smagin Vladimir Anatol'EvichMardanov Andrej VladimirovichBonch-Osmolovskaja Elizaveta AleksandrovnaRavin Nikolaj Viktorovich

(73)

Holder

Uchrezhdenie Rossijskoj Akademii Nauk Tsentr Ran

THERMOSTABLE DNA-LIGASE OF THERMOCOCCUS ARCHAEA, METHOD FOR PRODUCING THEREOF AND DNA NUCLEOTIDE SEQUENCE CODING SUCH DNA-LIGASE Russian patent published in 2010 - IPC C12N15/00 

Abstract RU 2405823 C2

FIELD: medicine.

SUBSTANCE: invention represents recombinant thermostable DNA-ligase Thermococcus sp 1519 exhibiting ligase activity in the presence of magnesium ions, in a wide range of pH values (7.0-10.7) and sodium chloride concentrations (25-150 mM). Such ligase consists of 559 amino acids, has mass of 63 kDa determined by polyacryl gel electrophoresis, and contains amino acid sequence SEQ ID NO:2 reproduced of recombinant strain E.coli. Also the invention refers to DNA coding said ligase, and a method for producing ligase.

EFFECT: invention allows producing new DNA-ligase with the improved properties.

3 cl, 3 dwg, 5 ex

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RU 2 405 823 C2

Authors

Smagin Vladimir Anatol'Evich

Mardanov Andrej Vladimirovich

Bonch-Osmolovskaja Elizaveta Aleksandrovna

Ravin Nikolaj Viktorovich

Dates

2010-12-10—Published

2009-01-29—Filed

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