FIELD: medicine.
SUBSTANCE: amplification of target products in carried out in special DNA thermocycler, provided with a special reaction thermal unit. Said thermocycler provides in reaction vessels, which are represented by standard polypropylene test tubes, temperature ingredients, oriented at angle to direction of gravitation action. Time of said amplification constitutes 1-5 minutes.
EFFECT: invention makes it possible to accelerate PCN by means of convection and simplify its carrying out preserving high specificity.
4 cl, 2 dwg, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF AMPLIFYING SPECIFIC NUCLEIC ACID FRAGMENTS THROUGH RECURRENT CHAIN REACTION | 2007 |
|
RU2414510C2 |
| METHOD OF STANDARDISATION OF CONVECTION POLYMERASE CHAIN REACTION VALUES WITH DIRECT FLUORESCENCE REGISTRATION OF REACTION PRODUCT ACCUMULATION | 2010 |
|
RU2423685C1 |
| METHOD FOR DIAGNOSING PORCINE ENZOOTIC PNEUMONIA BY POLYMERASE CHAIN REACTION | 2018 |
|
RU2702170C1 |
| METHOD AND KIT FOR DETECTING CAMPYLOBACTER BACTERIA IN PATIENTS WITH INFLAMMATORY INTESTINAL DISEASES | 2019 |
|
RU2732923C1 |
| METHOD DETECTION AND EVALUATION OF BIOTYPE, SEROGROUP AND TOXIGENICITY OF CHOLERA AGENT AND RELATED KIT | 2007 |
|
RU2360972C1 |
| DIAGNOSTIC TECHNIQUE OF 35DELG (RS80338939) MUTATION OF GJB2 GENE | 2020 |
|
RU2739889C1 |
| DIAGNOSTIC TECHNIQUE FOR THE 167DELT (RS80338942) MUTATION OF THE GJB2 GENE | 2020 |
|
RU2739943C1 |
| METHOD FOR DIAGNOSING MUTATION C.-23+1G>A (RS80338940) OF THE GJB2 GENE | 2020 |
|
RU2746055C1 |
| OLIGONUCLEOTIDE PRIMERS, FLUORESCENT PROBE AND METHOD FOR RECOGNISING RNA OF IBARAKI DISEASE VIRUS BY REAL-TIME POLYMERASE CHAIN REACTION | 2013 |
|
RU2540142C2 |
| METHOD OF QUANTITATIVE DETERMINATION OF FIXED RABIES VIRUS "MOSKVA 3253" | 2012 |
|
RU2511440C2 |
