FIELD: medicine.
SUBSTANCE: invention relates to biotechnology and represents stable and soluble scFv-antibody and Fab-fragment, specific with respect to TNFα, which contain specific sequences of light chain and heavy chain, optimised as to stability, solubility, in vitro and in vivo TNFα binding and low immunogenity. Also claimed are antibody-coding DNA-sequence, vectors, host-cell, as well as method of obtaining antibody with application of said cells. In addition claimed are therapeutic and diagnostic compositions and stable water pharmaceutically ready form based on antibodies, as well as method of treating TNFα-associated disease.
EFFECT: invention can be efficiently used for diagnostics and treatment of TNFα-associated disorders.
61 cl, 18 dwg, 5 tbl, 5 ex
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