STABLE AND SOLUBLE ANTIBODIES INHIBITING TNFα Russian patent published in 2015 - IPC C07K16/24 A61K39/395 C12N15/13 C12N15/63 

FIELD: chemistry.

SUBSTANCE: invention relates to biochemistry, particularly to an antibody or a fragment of said antibody, which specifically binds with human TNFα, wherein the antibody or antibody fragment contains a human heavy chain framework region sequence and a CDR sequence, derived from a rabbit antibody, as well as to a composition for treating TNFα-mediated diseases, which contains said antibody or fragment. The invention also discloses isolated nucleic acid which codes a heavy chain variable region and a light chain variable region of said antibody or fragment thereof, as well as an expression vector containing the same, and a host cell containing said vector. The invention also discloses use of said antibody or antibody fragment to obtain a medicinal agent for treating or preventing a human TNFα-mediated disease, as well as use in a method of treating or preventing a TNFα-mediated disease.

EFFECT: invention provides effective treatment or prevention of a human TNFα-mediated disease with antibodies which are optimised with respect to stability, solubility, in vitro and in vivo binding with TNFα and low immunogenicity.

24 cl, 12 dwg, 6 tbl, 6 ex