FIELD: chemistry.
SUBSTANCE: invention relates to purification of various gamma-carboxylated olypeptide forms with application of ion-exchange chromatography. In particular, in accordance with invention, claimed is method of purification of polypeptide, which has desirable content of gamma-carboxyglutaminic acid, from sample, containing mixture of said polypeptide versions, which have different content of gamma-carboxyglutaminic acid, with the claimed method including stages: (a) loading said sample on anion-exchange chromatographic material; (b) elution of said polypeptide with application of solution with pH lower than 9.0, containing at least one salt, selected from ammonium acetate, ammonium chloride and sodium acetate; and (c) selection of fraction, obtained after said elution, with polypeptides in said fraction having desired content of gamma-carboxyglutaminic acids.
EFFECT: claimed is method of purifying polypeptide, which has desirable content of carboxyglutaminic acid.
8 cl, 13 dwg, 6 tbl, 7 ex
