FIELD: medicine.
SUBSTANCE: basic, mass and standard procedure for bovine tuberculosis diagnosing is a mammal tuberculin protein purified derivate skin test. A method for producing a mycobacterial antigen involves removing free lipids of mycobacterial cells with acetone; destructing mycobacterial cell walls by dimethyl sulfoxide (DMSO) incubation in a sixfold volume at 37°C. The prepared supernatant is dialysed for 3 days against distilled water After the dialysis, a supernatant is kept at plus 4°C for 3 months and separated from the precipitation.
EFFECT: tests have shown high specificity of the prepared (DMSO-m) antigens and usability in lifetime differential diagnostics of bovine tuberculosis by immune-enzyme assay of blood serums.
1 dwg, 1 tbl, 3 ex
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