FIELD: chemistry.
SUBSTANCE: clone of mouse hybridome cells called "S148" is obtained by merging splenic lymphocytes of a mouse which is immunised by human recombinant protein SURF-6, which is merged with glutathione S-transferase which is expressed and extracted from E. coli cells, with cells of mouse myeloma of the line P3X63-Ag8.653 in ratio of 3:1 using a 50% solution of polyethylene glycol with molecular weight 1450. The obtained hybridome secretes the monoclonal antibody S148, which is specific towards human, mouse or rat nucleolar protein SURF-6, having an established amino acid sequence.
EFFECT: disclosed monoclonal antibody S148 enables to detect nucleolar protein SURF-6 in mammal cells using immunocytochemistry, immunoblotting and immunoprecipitation techniques.
8 dwg, 7 ex
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