METHOD OF DETECTING PROTEINS IN DIFFERENT CELL TYPES OF MAMMALS AND HUMAN USING FLUORESCEIN-5-ISOTHIOCYANATE AT MICROSCOPIC LEVEL Russian patent published in 2015 - IPC C12N5/07 C07K14/435 G01N33/48 G01N33/68 

FIELD: biotechnology.

SUBSTANCE: method of detection of intracellular proteins using fluorescein-5-isothiocyanate (FITC) in various types of mammalian cells is provided, which are characterised by different levels of the synthetic process, using the method of confocal laser microscopy. The mammalian cells are fixed by paraformaldehyde (PFA) preventing the extraction of proteins in subsequent stages of staining, permeabilised with detergent, then stained for 2 h with FITC in a concentration of 1 mcg/ml. The cells are enclosed in Mowiol 4-88 adding DABCO (1,4-diazabicyclo[2.2.2] octane). The resulting preparations are used to analyse the localisation and the content of the protein component of the cells by confocal microscopy.

EFFECT: invention enables to obtain information and to investigate the location of proteins in the cells and to carry out a comparative analysis of protein content in cells with different levels of metabolism.

7 dwg, 7 ex