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2 422 512

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IPC

C12N1/21(2006-01-01)

C12N15/12(2006-01-01)

C12R1/19(2006-01-01)

(21) (22)

Application

2010105151/10, 2010-02-16

(24)

Start date

2010-02-16

(22)

Actual filing date

2010-02-16

(45)

Published

2011-06-27

(72)

Inventor

Severin Evgenij SergeevichLaurinavichjute Daniela KestuchePozdnjakova Natal'Ja VladimirovnaFedorov Aleksej NikolaevichSharapova Ol'Ga AndreevnaJurkova Marija SergeevnaSeverin Sergej Evgen'Evich

(73)

Holder

Avtonomnaja Nekommercheskaja Organizatsija Molekuljarnoj Diagnostiki"

Escherichia coli BL21(DE3)/pAFP11D3 STRAIN - PRODUCER OF 404 TO 609 AMINO ACID FRAGMENT OF HUMAN ALPHA-FETOPROTEIN Russian patent published in 2011 - IPC C12N1/21 C12N15/12 C12R1/19

Abstract RU 2422512 C1

FIELD: medicine.

SUBSTANCE: invention refers to biotechnology, particularly to recombinant production of human oncofetal proteins, and can be used for producing a 404 to 609 amino acid fragment of human alpha-fetoprotein. The Escherichia coli BL21(DE3)/pAFP11D3 strain - a producer of the 404 to 609 amino acid fragment of human alpha-fetoprotein is produced by Escherichia coli cell transformation of the BL21(DE3) strain of plasmid DNA pAFP11D3.

EFFECT: invention allows reducing potential human viral contamination when using a recombinant method to produce said alpha-fetoprotein fragment exhibiting affinity to an alpha-fetoprotein receptor and exposed to receptor-mediated endocytosis.

2 cl, 7 dwg

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RU 2 422 512 C1

Authors

Severin Evgenij Sergeevich

Laurinavichjute Daniela Kestuche

Pozdnjakova Natal'Ja Vladimirovna

Fedorov Aleksej Nikolaevich

Sharapova Ol'Ga Andreevna

Jurkova Marija Sergeevna

Severin Sergej Evgen'Evich

Dates

2011-06-27—Published

2010-02-16—Filed