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2 617 943

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IPC

C12N15/12(2006-01-01)

C12N15/81(2006-01-01)

C12N1/19(2006-01-01)

C12P21/02(2006-01-01)

C12R1/865(2006-01-01)

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Application

2015129623, 2015-07-17

(24)

Start date

2015-07-17

(22)

Actual filing date

2015-07-17

(45)

Published

2017-04-28

(72)

Inventor

Benevolenskij Sergej VladimirovichVavilova Ekaterina AleksandrovnaDudich Igor VyacheslavovichZatsepin Sergej SergeevichMorozkina Elena VladimirovnaSemenkova Lidiya NikolaevnaOstroumov Yurij Igorevich

(73)

Holder

Obshchestvo S Ogranichennoj Otvetstvennostyu Predpriyatie Tekhnologii" Tekhnologii")

RECOMBINANT DNA FRAGMENT, ENCODING HUMAN ALPHA-FETOPROTEIN (AFP), CONTAINING "SLOW" LEUCINE-ENCODING CODONS, EXPRESSION PLASMID, CONTAINING SAID FRAGMENT, SACCHAROMYCES CEREVISIAE CELL, TRANSFORMED WITH SAID PLASMID, AND METHOD FOR OBTAINING HUMAN AFP Russian patent published in 2017 - IPC C12N15/12 C12N15/81 C12N1/19 C12P21/02 C12R1/865

Abstract RU 2617943 C2

FIELD: medicine.

SUBSTANCE: invention relates to field of biotechnology, can be used for obtaining human alpha-fetoprotein (AFP) with application of strains of Saccharomyces cerevisiae yeasts, containing recombinant AFP gene. Obtained are: recombinant human AFT, containing so-called "slow" codons, encoding leucine in conservative AFP sections, recombinant plasmid and Saccharomyces cerevisiae cell for expression and secretion of said recombinant AFP respectively.

EFFECT: invention makes it possible to increase production of active AFP.

9 cl, 16 dwg, 3 tbl, 6 ex

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RU 2 617 943 C2

Authors

Benevolenskij Sergej Vladimirovich

Vavilova Ekaterina Aleksandrovna

Dudich Igor Vyacheslavovich

Zatsepin Sergej Sergeevich

Morozkina Elena Vladimirovna

Semenkova Lidiya Nikolaevna

Ostroumov Yurij Igorevich

Dates

2017-04-28—Published

2015-07-17—Filed