METHOD OF RECOVERY LOW-MOLECULAR NUCLEAR RIBONUCLEOPROTEIDS (RNP) FROM TISSUES AND ORGANS OF MAMMALS Russian patent published in 2011 - IPC C12P19/30 C12P21/00 

FIELD: medicine.

SUBSTANCE: neutral detergent Cevtalone is used for recovery and purification of a low-molecular ribonucleoproteid fraction from DNA and high-molecular RNA. Cevtalone forms from water-insoluble salts with DNA and RNA thereby enables consistent precipitation of DNA and high-molecular RNA from the solution. As a result, the solution contains the low-molecular ribonucleoproteid fraction which then precipitated with Cevtalone. The produced precipitate is dissolved in ethanol for pure ribonucleoproteid precipitation. Cevtalone salts of nucleic acids are resistant to ribonuclease enzymes thereby the given method can be staged with great time intervals (up to several days) without loss of biological properties of recovered ribonucleoproteids. The given method of recovery allows to producing high-purity low-molecular ribonucleoproteids (the ribonucleoproteid contents in the prepared samples is 90 %) in preparative amounts.

EFFECT: recovery of low-molecular nuclear ribonucleoproteid from tissues of mammals with using non-toxic and low-cost reagents.

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