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IPC

C12N15/10(2006-01-01)

C12P19/34(2006-01-01)

C07H21/04(2006-01-01)

(21) (22)

Application

2009112677/10, 2009-04-06

(24)

Start date

2009-04-06

(22)

Actual filing date

2009-04-06

(45)

Published

2011-01-10

(72)

Inventor

Laktionov Pavel PetrovichIl'Ichev Aleksandr AlekseevichBondar' Anna AleksandrovnaCherepanova Anna Vital'EvnaSkvortsova Tat'Jana Ehval'DovnaKarpenko Larisa IvanovnaChernonosova Vera SergeevnaMorozkin Evgenij SergeevichBazhan Sergej IvanovichOreshkova Svetlana FedorovnaNechaeva Elena AvgustovnaDrozdov Il'Ja GennadievichVlasov Valentin Viktorovich

(73)

Holder

Uchrezhdenie Rossijskoj Akademii Nauk Institut Khimicheskoj Biologii I Fundamental'Noj Meditsiny Sibirskogo Otdelenija Ran So Ran)Federal'Noe Gosudarstvennoe Uchrezhdenie Nauki Gosudarstvennyj Nauchnyj Tsentr Virusologii I Biotekhnologii Federal'Noj Sluzhby Po Nadzoru V Sfere Zashchity Prav Potrebitelej I Blagopoluchija Cheloveka Gnts Vb Rospotrebnadzora)

METHOD FOR PRODUCTION OF PLASMID DNA FROM BACTERIAL CELLS Russian patent published in 2011 - IPC C12N15/10 C12P19/34 C07H21/04

Abstract RU 2408729 C2

FIELD: medicine.

SUBSTANCE: method includes alkaline lysis of bacterial cells and depositing of cellular walls by centrifugation. Afterwards supernatant is filtered, and RNA admixture is removed from produced filtrate by means of filtrate treatment with RNA-ase. Then plasmid DNA is deposited with isopropyl alcohol. Then divisional fractioning of plasmid DNA is carried out with ethyl alcohol, when first ethyl alcohol is added to solution of plasmid DNA to concentration of 45-53%, then ethyl alcohol is added to concentration of 55-58%, and in the end ethyl alcohol is added to concentration of 60-67%. Afterwards lipopolysaccharide remains are removed with the help of reverse-phase sorbent with hydrophobised surface.

EFFECT: improved cleanliness and yield of plasmid DNA.

3 cl, 1 tbl, 4 ex

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RU 2 408 729 C2

Authors

Laktionov Pavel Petrovich

Il'Ichev Aleksandr Alekseevich

Bondar' Anna Aleksandrovna

Cherepanova Anna Vital'Evna

Skvortsova Tat'Jana Ehval'Dovna

Karpenko Larisa Ivanovna

Chernonosova Vera Sergeevna

Morozkin Evgenij Sergeevich

Bazhan Sergej Ivanovich

Oreshkova Svetlana Fedorovna

Nechaeva Elena Avgustovna

Drozdov Il'Ja Gennadievich

Vlasov Valentin Viktorovich

Dates

2011-01-10—Published

2009-04-06—Filed