INSTANT DIAGNOSTIC TECHQNIUE FOR DIABETIC NEPHROPATHY STAGES Russian patent published in 2011 - IPC G01N33/48 

FIELD: medicine.

SUBSTANCE: instant diagnostic technique for diabetic nephropathy stages involving detecting a renal structure consists that in the fact that a glass plate 12×6 cm is coated with staggered strips 4-5 mm each of 0.01-0.02 ml of a biological test system containing 0.2% aqueous amino acids: glutamic acid, valine, tyrosine, serine, tryptophan, leucine, arginine taken in equal proportions; 0.25% DNA sodium salt; 25% aqueous magnesia sulphate in the ratio 2:1:2; the plate is placed in a projection of a kidney along a spinal column between the XII thoracic and III lumbar vertebras, kept for 4-5 minutes, then the preparation is dried at temperature +18-20°C for 2-3 minutes, analysed under a microscope in polarised light with a quartz compensator, and if observing: single coarse-grained cells and/or fine-grained cells, rarefied subparallel aggregates with spiral-form inclusions in the sample, the stage I of diabetic nephropathy is stated; the presence of fine-grained cells, rounded aggregates with crystalloid inclusions, rarefied subparallel aggregates with relic spiral-form inclusions shows the stage II of diabetic nephropathy, while the presence of undifferentiated rarefication and relict sites of spiral-form aggregates enables to diagnose the stage III of diabetic nephropathy.

EFFECT: technique is easy to implement, high-quality, eliminates invasiveness.

8 dwg, 3 ex