FIELD: food industry.
SUBSTANCE: method envisages raw material milling into 700±40 mcm fraction. Then the raw materials undergoes treatment with ultrasound with frequency 18-24 kHz and intensity 50-100 W/cm2 during 0.5-2 min at a temperature of 20-25°C. Then suspension is prepared wherein the ratio of milled raw material to water is 1:3 with the produced mass subsequently maintained during 12 h at a temperature of 20-25°C. Then one proceeds with enzyme hydrolysis using preparations of cellulase in an amount of 2 KMTsS units/ g of reference starch, proteinase in an amount of 1 PS init/ g of reference starch and amylosubtiline in an amount of 0.5 AS init/ g of reference starch and subsequent fermentation of the produced hydrolysate into citric acid.
EFFECT: invention enables improvement of the quality of starch-containing raw materials hydrolysates for citric acid biosynthesis due to enhanced efficiency of polysaccharides removal from vegetal raw material.
2 tbl, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| STRAIN Aspergillus niger-PRODUCER OF CITRIC ACID | 2013 |
|
RU2558228C2 |
| METHOD FOR PREPARING NUTRIENT MEDIUM FOR YEAST CULTURING | 2002 |
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| FOOD SUPPLEMENT FOR HYDROLYSIS OF PLANT RAW MATERIALS | 2005 |
|
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| METHOD OF COMPLEX PROCESSING GRAIN RAW MATERIAL FOR ALCOHOL AND FEED PRODUCTS ENRICHED IN LYSINE | 2009 |
|
RU2396007C1 |
| METHOD FOR PREPARING VEGETABLE RAW TO MICROBIOLOGICAL CONVERSION | 2000 |
|
RU2202606C2 |
| METHOD OF GROWING YEAST | 2013 |
|
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| METHOD OF OBTAINING NUTRITIONAL MEDIUM FOR CARRYING OUT MICROBIAL LYSINE SYNTHESIS | 2009 |
|
RU2412242C2 |
| METHOD OF PRODUCTION OF ETHYL ALCOHOL | 2001 |
|
RU2199586C2 |
| METHOD OF PRODUCING FEED ADDITIVE FROM GRAIN RAW MATERIAL | 2011 |
|
RU2466552C2 |
| MALTOSE SYRUP PRODUCTION METHOD | 2009 |
|
RU2425892C2 |
