METHOD OF DETECTING 2-DIMETHYLAMINO-1,3-BIS-(PHENYLSULPHONYLTHIO)PROPANE IN BIOLOGICAL MATERIAL Russian patent published in 2011 - IPC C07C381/04 G01N33/48 G01N30/02 

FIELD: chemistry.

SUBSTANCE: analysed sample is infused with an organic insulating agent. The obtained extract is purified through chromatography on a column of silica gel L 40/100 µ, while performing elution with a mixture of organic solvents. The analysed substance is determined using a chromatographic technique using a mobile phase which contains hexane, dioxane and propanol-2. The organic insulating agent is toluene. The toluene extract is dehydrated with anhydrous sodium sulphate. During the purification process, hexane is first passed through the column and elution is then carried out with a hexane-dioxane-propanol-2 solvent mixture (8:3:0.6 by volume). Eluate fractions containing the analysed substance are merged. The eluent is evaporated. The residue is dissolved in the hexane-dioxane-propanol-2 solvent mixture (15:5:1 by volume) and detection is carried out using high-performance liquid chromatography (HPLC) in a 64×2 mm column filled with Silasorb-600 sorbent using a hexane-dioxane-propanol-2 mobile phase (15:5:1 by volume) and a UV detector.

EFFECT: high accuracy and sensitivity of analysis.

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