FIELD: medicine.
SUBSTANCE: method of microbiological synthesis of mature human interferon alpha-2 is implemented by cultivation of Saccharomyces cerevisiae yeast containing inactivating mutation in a structural gene of proteinase YPS1 and/or additional genes of proteinase KEX2. A recombinant method is used to produce Saccharomyces cerevisiae yeast strains capable to secrete mature human interferon alpha-2 in a culture medium.
EFFECT: invention allows increasing production of mature human interferon alpha-2 ensured by reduced degradation of secreted interferon by yeast proteinase YPS1 gene inactivation, and by improved effectiveness of processing of secreted interferon precursor by increasing native or secreted proteinase KEX2 expression.
9 cl, 1 tbl, 33 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF PRODUCING MATURE HUMAN INTERFERON ALPHA-2 USING SACCHAROMYCES CEREVISIAE YEAST AND HUMAN INTERFERON ALPHA-2 PRODUCER STRAIN (VERSIONS) | 2011 |
|
RU2446172C1 |
| HYBRID PROTEIN HAVING PROLONGED ACTION, BASED ON RECOMBINANT HUMAN INTERFERON ALPHA-2 (VARIANTS), METHOD OF ITS PRODUCTION AND STRAIN OF Saccharomyces cerevisiae FOR IMPLEMENTING THIS METHOD (VERSIONS) | 2013 |
|
RU2515913C1 |
| METHOD FOR MICROBIOLOGICAL SYNTHESIS OF SECRETED HUMAN SOMATOTROPIN AND YEAST STRAIN SACCHAROMYCES CEREVISIAE - SECRETED HUMAN SOMATOTROPIN PRODUCER | 2011 |
|
RU2460795C1 |
| METHOD OF ENGINEERING Saccharomyces cerevisiae YEAST STRAINS - STABLE HUMAN SOMATOTROPIN PRODUCERS, Saccharomyces cerevisiae YEAST STRAIN - STABLE HUMAN SOMATOTROPIN PRODUCER (VERSIONS) | 2009 |
|
RU2420567C1 |
| HYBRID PROTEIN (VERSIONS), BACTERIAL STRAIN ESCHERICHIA COLI - HYBRID PROTEIN PRODUCER (VERSIONS) AND METHOD FOR PRODUCING METHIONINE-FREE HUMAN INTERFERON ALPHA-2 | 2011 |
|
RU2453604C1 |
| METHOD TO PRODUCE PROTEIN E7-HSP70 AND YEAST STRAIN Saccharomyces cerevisiae FOR ITS REALISATION | 2012 |
|
RU2489481C1 |
| FUSION PROTEIN, ESCHERICHIA COLI STRAIN BEING FUSION PROTEIN PRODUCER AND METHOD FOR PRODUCING METHIONINE-FREE HUMAN INTERFERON ALPHA-2b OF SUCH FUSION PROTEIN | 2010 |
|
RU2441072C1 |
| MICROBIOLOGICAL SYNTHESIS METHOD OF TARGET RELEASED PROTEIN IN YEAST Saccharomyces cerevisiae | 2012 |
|
RU2502805C1 |
| METHOD OF PRODUCING RECOMBINANT SPIDER-WEB PROTEIN, FUSED PROTEIN, RECOMBINANT DNA, EXPRESSION VECTOR, HOST CELL AND PRODUCER STRAIN | 2010 |
|
RU2451023C1 |
| RECOMBINANT PLASMID DNA DETERMINING SYNTHESIS OF HUMAN FIBROBLAST INTERFERON IN YEAST CELLS, METHOD OF ITS CONSTRUCTION AND YEAST STRAIN SACCHAROMYCES CEREVISIAE AS PRODUCER OF HUMAN FIBROBLAST INTERFERON | 1998 |
|
RU2180003C2 |
