FIELD: medicine.
SUBSTANCE: construction of recombinant plasmid DNAs containing a structured gene of human interferon alpha-2b under control of a sensitive promoter enables producing Escherichia coli strain to enable synthesis in the form of an insoluble precursor protein of mature methionine-free human interferon alpha-2b. In the precursor protein, a sequence of mature methionine-free human interferon alpha-2b is fused with a sequence of SUMO (SMT3) Saccharomyces cerevisiae yeast protein. What is produced is the Escherichia coli strain providing biosynthesis of ULP275 proteinase for enzymatic processing of the precursor protein of methionine-free interferon. What is presented is a method for producing methionine-free interferon which provides carrying out the coupled processes of renaturation, disulphide link formation and enzymatic segregation of methionine-free human interferon alpha-2b from the precursor protein under ULP275 proteinase.
EFFECT: invention provides the range of products for preparing human methionine-free IFN-α2.
3 cl, 14 ex
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RU2604796C1 |
| METHOD FOR PREPARING PROTEINASE Ulp275 | 2011 |
|
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| PLASMID VECTOR PRH15A FOR PRODUCING METHIONINE-FREE INTERFERON ALPHA-2B, BACTERIAL STRAIN ESCHERICHIA COLI BL21 DE3 - PRODUCER OF METHIONINE-FREE INTERFERON ALPHA-2B AND METHOD FOR PRODUCING METHIONINE-FREE INTERFERON ALPHA-2B | 2017 |
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| METHOD FOR PRODUCING OF HUMAN METHIONINE-FREE INTERFERON-ALPHA2b | 2009 |
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RU2432401C2 |
| METHOD OF OBTAINING BIOLOGICALLY ACTIVE RECOMBINANT PROTEINS | 2022 |
|
RU2807615C2 |
| METHOD OF MICROBIOLOGICAL SYNTHESIS OF MATURE HUMAN INTERFERON ALPHA-2, Saccharomyces cerevisiae STRAIN - MATURE HUMAN INTERFERON ALPHA-2 PRODUCER (VERSIONS) | 2010 |
|
RU2427645C1 |
| HYBRID PROTEIN HAVING PROLONGED ACTION, BASED ON RECOMBINANT HUMAN INTERFERON ALPHA-2 (VARIANTS), METHOD OF ITS PRODUCTION AND STRAIN OF Saccharomyces cerevisiae FOR IMPLEMENTING THIS METHOD (VERSIONS) | 2013 |
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RU2515913C1 |
| RECOMBINANT PLASMID PFM-IFN-17 PROVIDING EXPRESSION OF INTERFERON ALPHA-2B OF HUMAN, RECOMBINANT PLASMID PFM-AP, PROVIDING EXPRESSION OF ENZYME METHIONINE-AMINOPEPTIDASE E COLI, BIPLASMID STRAIN ESCHERICHIA COLI FM-IFN-AP (PFM-IFN-17, PFM-AP) - PRODUCER (MET-) OF RECOMBINANT INTERFERON ALPHA -2B OF HUMAN | 2016 |
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| METHOD OF PRODUCING MATURE HUMAN INTERFERON ALPHA-2 USING SACCHAROMYCES CEREVISIAE YEAST AND HUMAN INTERFERON ALPHA-2 PRODUCER STRAIN (VERSIONS) | 2011 |
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RU2446172C1 |
