FIELD: medicine.
SUBSTANCE: constructing a recombinant plasmid DNA containing structural gene of human interferon alpha-2b or alpha-2a under control of regulated promoter enables producing bacterial strains Escherichia coli providing synthesis of insoluble precursor proteins of mature methionine-free human interferons alpha-2b or alpha-2a. The precursor proteins of the sequence of the mature methionine-free human interferons alpha-2b or alpha-2a are fused with a sequence of SUMO (SMT3) protein of yeast Saccharomyces cerevisiae. What is produced is the bacterial strain Escherichia coli providing biosynthesis of ULP275 proteinase for enzymatic processing of the precursor protein of methionine-free interferons. What is developed is a method for producing methionine-free interferons which provides carrying out the coupled renaturation processes, disulphide link formation and enzymatic segregation of methionine-free human interferons alpha-2b or alpha-2a from the precursor protein under ULP275 proteinase.
EFFECT: method improvement.
6 cl, 20 ex
| Title | Year | Author | Number |
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|
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| METHOD OF MICROBIOLOGICAL SYNTHESIS OF MATURE HUMAN INTERFERON ALPHA-2, Saccharomyces cerevisiae STRAIN - MATURE HUMAN INTERFERON ALPHA-2 PRODUCER (VERSIONS) | 2010 |
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| METHOD FOR PRODUCING OF HUMAN METHIONINE-FREE INTERFERON-ALPHA2b | 2009 |
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| HYBRID PROTEIN BASED ON L-ASPARAGINASE Wolinella succinogenes, STRAIN Escherichia coli - PRODUCER OF HYBRID PROTEIN (VERSIONS) AND METHOD OF PRODUCING OF HYBRID PROTEIN WITH ANTICANCER ACTIVITY | 2014 |
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RU2562166C1 |
| METHOD OF PRODUCING MATURE HUMAN INTERFERON ALPHA-2 USING SACCHAROMYCES CEREVISIAE YEAST AND HUMAN INTERFERON ALPHA-2 PRODUCER STRAIN (VERSIONS) | 2011 |
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| METHOD OF COMMERCIAL E.coli STRAIN CULTURE PREPARED OF BL21(DE3) STRAIN CARRYING POLYMERASE T7 RNA GENE UNDER CONTROL OF lacUV5 PROMOTER WITH INCREASED BIOMASS SYNTHESIS AND HIGHER END PROTEIN YIELD IN INCLUSION BODIES | 2011 |
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