RECOMBINANT PLASMID DNA DETERMINING SYNTHESIS OF HUMAN FIBROBLAST INTERFERON IN YEAST CELLS, METHOD OF ITS CONSTRUCTION AND YEAST STRAIN SACCHAROMYCES CEREVISIAE AS PRODUCER OF HUMAN FIBROBLAST INTERFERON Russian patent published in 2002 - IPC

FIELD: biotechnology, genetic engineering, micribiological and medicinal industry. SUBSTANCE: method involves transformation of the strain of yeast p4-1-GRF18 with recombinant plasmid DNA constructed in vitro and containing encoding moiety of human beta-interferon gene (with exception of region encoding signal peptide) and the strain p4-1-GRF18(pHBI) is obtained that is a producer of human fibroblast interferon. The prepared strain of yeast Saccharomyces cerevisiae p4-1-GRF18(pHBI) is deposited in All- Russian collection of industrial microorganisms at number VKPM-Y-2286. EFFECT: enhanced level of synthesis of human fibroblast interferon. 3 cl, 2 dwg