FIELD: medicine.
SUBSTANCE: method involves Aeromonas sobria vaccine strain seeding, cultivation and inactivation. Aeromonads are cultivated in a liquid nutrient medium of a Hottinger broth in a fermenter for 4-6 hours at temperature 26±1°C, immediately after seeding, an oxidation-reduction potential of the cultural liquid is reduced to (-40) - (-100) mV. Thereafter, before termination of a cultivation process, pO2 in the cultural fluid is kept at 20-30 % from air oxygen saturation, pH of the cultural fluid is regulated at 7.1-7.3 pH units, and fractional glucose supply is carried out in doses to the concentration 0.25-0.35 % with glucose limitation of aeromonad growth.
EFFECT: method allows higher yield of the vaccine with reduced time of preparation.
4 tbl, 5 ex
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