FIELD: biotechnology, veterinary science. SUBSTANCE: method involves submerged culturing pasteurella cells on nutrient medium based on dried protein concentrate hydrolysate under control of pH value, partial pressure of oxygen dissolved in cultural fluid and batch glucose feeding. Obtained culture is concentrated, packaged into flasks and lyophilized (live vaccines) or inactivated, mixed with adjuvant and packaged into flasks (inactivated vaccines) or used for preparing mixed and polyvalent vaccines. Method ensures to change food meet raw as a base of nutrient medium for hydrolysate of food production waste in preparing vaccines against pasteurellosis in agriculture animals and poultry. Method can be used for industrial production of live and inactivated vaccines against pasteurellosis in animals and poultry. EFFECT: decreased cost, improved stability and standard of biological properties of vaccine preparations. 1 tbl, 5 ex
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Authors
Dates
1999-04-27—Published
1998-06-09—Filed