FIELD: medicine.
SUBSTANCE: there is produced a two-cassette genetic plasmid-based constraint pBud-VEGF-GDNF containing DNA fragments coding VEGF and GDNF. Mononuclear cells are recovered from human blood. The recovered cells are genetically modified by pBud-VEGF-GDNF. The genetically modified cells are cultivated, concentrated, purified from a culture medium, resuspended. The genetically modified cells are analysed for quantity, concentration, viability, and the analysis results are used to calculate an optimum therapeutic dose of the genetically modified cells which is a preparation of the genetically modified cells.
EFFECT: invention allows producing a preparation with substantially improved medicinal properties.
2 cl
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