DIAGNOSTIC TECHNIQUE FOR SECONDARY IMMUNODEFICIENT DISEASE IN PULMONARY TUBERCULOSIS Russian patent published in 2014 - IPC G01N33/53 

FIELD: medicine.

SUBSTANCE: prescribing the specific chemotherapy is preceded by the immunological examination of peripheral blood of the patients suffering pulmonary tuberculosis and the determination of cell, humoral and molecular-genetic parameters of the patient's immune status. The peripheral blood CD4⁺CD25^-Foxp3⁺ regulatory T-cell count more than 2.6%, the peripheral blood CD4⁺CD25⁺Foxp3⁺ regulatory T-cell count more than 5.1%, the peripheral blood CD3⁺CD4^- CD25⁺ regulatory T-cell count more than 0.2%, the peripheral blood CD4⁺CD25⁺Foxp3^- regulatory T-cell/activated T-helper count less than 25.5%, the peripheral blood CD3⁺CD4⁺CD25^- T-helper count less than 35.4%, the peripheral blood γδT-lymphocyte count less than 4.9%, the peripheral blood CD45R0⁺ memory T-cell count less than 8.9%; 1L-2 less than 22.3 pg/ml, IL-4 more than 40.0 pg/ml, IL-10 more than 25.3 pg/ml, TGFβ more than 1109.0 pg/ml in a combination with the carriage of the allele C and genotype GG T-330G of IL2 gene and the allele A and genotype AA C-592A of IL10 gene, of the allele T and genotype TT C-509T of TGFB gene, and the allele T and genotype TT C-590T of IL4 gene, secondary immunodeficient disease is diagnosed.

EFFECT: invention enables the well-timed diagnosing of the immune system defects in the patients with pulmonary tuberculosis and provides the measures of therapeutic modality correction with using the advanced method of immunomodulatory therapy.

6 dwg, 4 tbl, 1 ex