METHOD FOR PREPARING SECTIONS OF FRESH FROZEN LYMPH NODE TISSUE Russian patent published in 2011 - IPC G01N33/48 G01N1/28 

FIELD: medicine.

SUBSTANCE: sections are prepared by freezing a biopsy material plate in n-hexane cooled to temperature -95°C, and making sections in thickness no more than 7 mcm in a cryostat. The section is placed on a slide, drowned in an alcohol-aldehyde solution at room temperature (20-25°C) for 3 minutes, then washed in flowing water from a fixative for 30 seconds, stained with histological stains and enclosed by a cover glass consists under integumentary glass in the usual way. The preparations produced as described above preserve completely the structure of tissues and separate cells and allow describing lymphatic tumours according to the existing classifications. The method can be used in urgent histological examinations (intraoperative biopsies) as allows drawing up a conclusion decision for 20 minutes.

EFFECT: use of the method allows reducing working hours for the histological examinations required for diagnostics, in comparison with a conventional method, does not deteriorate the performed operations, and accelerates considerably drawing up the conclusion decisions.

3 dwg, 3 ex